The New Docetaxel E7080 Is Twice The Fun

The body bodyweight of each rat was measured weekly, and the dosage of drug or car administered was calculated determined by one of the most current body bodyweight measurement.

The liver and left tibia Docetaxel of each animal were fixed with fixative and used for further histomorphometric analysis, while the right tibia was freed of all soft tissue and wrapped in a layer of PARAFILM, apart from 5 mm of its proximal end, and fixed into a 15 ml BD Falcon Tube and then soaked in fixative. The tube cap was tightened before performing a u CT scan to measure the microstructural parameters. The right femurs were subjected to DEXA measurement for BMD and bone mineral content. To assess bone loss, rats right tibiae were ex vivo scanned at the end of drug treatment. A 6 mm uCT scan with an isotropic resolution of 18 um was made of the proximal tibia using an in vivo u CT scanner The CT scanner was calibrated, and a beam hardening correction algorithm was applied to all scans. One CT scan took 35 minutes. In this study, the reproducibility E7080 of all structural parameters was high, with a coefficient of variation of about 1%.

With this protocol, the gray levels of voxels near the trabecular surfaces are not included to ensure that the measurements are not affected by partial volume effects. All DEXA measurements were Docetaxel performed by the same investigator using the Norland pDEXA Sabre equipped with Sabre Research software. The interassay coefficient of variation for BMD and BMC was 1. 7%. The scanner was calibrated daily to a dual material standard according to the manufacturers recommendations, and the scanner performance was controlled by the quality assurance protocol of our laboratory. The right femurs were scanned using DEXA to determine BMC and BMD.

The baseline point was located on the cotton piece. Liver specimens were fixed in 10% buffered neutral paraformaldehyde solution, processed and embedded in paraffin. Thin paraffin sections were stained by hematoxylin and eosin.